Oct 20, 2014 in our study, the overall diagnostic accuracy of squash cytology was 95% when compared with histology as the gold standard, which correspond to or even better than some of the studies 11. Early morning urine should be avoided because of the poor morphological details shown by the cells exfoliated during the night and being exposed to urine for several hours. Cytology sample collection and preparation for veterinary. View squash cytology ppts online, safely and virusfree. Cytology samples can be collected from solid lesions by several techniques including. If cell pellet is not free of blood, do a second cytolyt wash 6. Ependymomas constitute 810 and % of central nervous system tumors in children and adults, respectively. In the cell, there are organelles which are suspended within an aqeuos medium and contained within plasmamembrane. How to grow roses from cuttings fast and easy rooting rose cuttings with a 2 liter soda bottle duration.
Find powerpoint presentations and slides using the power of, find free presentations research about squash cytology ppt. How to prepare a slide for a cytology evaluation youtube. Diagnosis of any pathology can be done with very few cells, but the more cells available. Sample preparation overview routine urine cytology 1. Role of diagnostic cytology, collection and preparation of material for cytodiagnosis, cytopreparatory techniques of serous effusions, fixation of cytology specimens, staining methods in cytology.
First introduced in 1930, central nervous system cns squash cytology csc has now been established as a method of intraoperative diagnosis of cns tumors. Collection of specimens and preparation methods eurocytology. Professormorsani college of medicine university of south florida health sciences. Mar 07, 2011 ultrastructure of animal and plant cells. Alternatively, cells can be harvested via a direct impressed iatrogenic material or fluid preparation s, via lavage or invivo exfoliation into an effusion. Squash preparation is a reliable, rapid and easy method and. Find powerpoint presentations and slides using the power of, find free presentations research about liquid based cytology ppt.
Mar 18, 20 squash preparation tissue is crushed between two slides and then stained similar to touch preparations, this method will give excellent cytology, although some cells may show some crush artifacts 10. Because of the microscopy requirements, options for preparing specimens are limited to. Organelles are tiny structures in the cytoplasm which perform various jobs for the cell. The technique has recently gained importance because. Analysis of squash smear cytology of ependymomas request pdf. Original article intraoperative squash cytology of central. Is the study of cells structures and their functions a cell is an organized independent mass of protoplasm nucleus and cytoplasm which makes the basic primary structure of an organism. Wholemounts, where an entire organism or structure is small enough or thin enough to be placed directly onto a microscope slide e. Ppt accuracy of intraoperative rapid diagnosis by squash. Several smear making methods and variations have been documented personal preference and teaching exposure influence the method of choice but the physical properties of the fna material including the concentration of hypocellular material or cyst fluid can also dictate the most useful method of triaging and smear making. The soft consistency of cns tissue is best suited for squash cytology, which in fact is a hindrance for frozen section. Introduction squash smear preparation fairly accurate, simple and reliable tool for rapid intraoperative diagnosis of central nervous system lesions.
All patients operated for cns mass lesions were included squash cytology reported by pathologists all were subjected for routine histopathological. How to take any sample containing cells, whether an aspirate from an organ or mass, or a fluid aspirate, and make a slide that can be evaluated in house or s. Though intraoperative squash smear cytology is well documented, grading is rarely described. Ultra structure of the cell is the fine structure of the cell as seen under electron microscope. If you are unable to view it properly please click here. Fiberoptic bronchoscopic biopsies yield very small bits of tissue, leading to high false negativity in lung cancer diagnosis, after paraffin embedding.
Cytology, or cytopathology, often requires the use of fine less than 20 gauge needle sampling to obtain cells to identify a pathological process. Squash preparation with experience, can yield excellent cytologic smears aspirated material is placed on the center of the slide a second slide is placed over the sample to form a cross. View and download powerpoint presentations on liquid based cytology ppt. Utility of squash smear cytology in fiberoptic bronchoscopic. A free powerpoint ppt presentation displayed as a flash slide show on id. Ideal for cutaneous or subcutaneous masses since it avoids surface contamination. Obtaining good quality samples for cytology in practice.
However, many of the comments that follow are relevant to all aspects of veterinary cytology specimen collection and preparation. Primary sclerosing cholangitis and brush cytology sampling. Processing cytology samples in the laboratory eurocytology. For this reason, as a general rule, we suggest routine storage of one or more of the biopsies in formalin while squash preparation cytology is undertaken, in order that histology may be performed subsequently should squash preparation cytology be inconclusive. I tried to influence them with trypsin for few minutes to break cell adherent proteins. I have problems to squash them corectly in order to be in one layer so i can easily take photo of mitotic cells. In our study, the overall diagnostic accuracy of squash cytology was 95% when compared with histology as the gold standard, which correspond to or even better than some of the studies 11. Squashpreparation cytology from nasopharyngeal masses in the. Smear making and special techniques for recovering and. Utility of squash smear cytology in intraoperative diagnosis. Intraoperative squash cytology is fairly accurate, reliable and cost effective method for rapid diagnosis of cns lesions. Histology is the study of the tissues of the body and of how these tissues are arranged to constitute organs. Cytology and cytological techniques yola powerpoint. The intraoperative cytology preparation was first introduced by eisenhardt and cushing in early 1930 and by badt in 1937.
Pdf intraoperative squash smear cytology in cns lesions. Squash preparation tissue is crushed between two slides and then stained similar to touch preparations, this method will give excellent cytology, although some cells may show some crush artifacts 10. Efforts should be made to detect early neoplastic changes that can. Cytology and cytological techniques yola xpowerpoint. Primary sclerosing cholangitis psc is associated with the development of cholangiocarcinoma in up to 10% of patients. Processing cytology samples in the laboratory processing cytology samples involves reception of the specimen and request form, preparation of slides for microscopic examination, staining, screening and reporting the slides. I got cells in one layer but there were no mitotic cells. Preparation of smears from aspirates squash prep method needle spread method blood smear method squash preparation with ppt sponsored links displaying powerpoint presentation on cytology and cytological techniques yola available to view or download. Professor asastent of department histology, cytology and embryology khodorovska alla 2. View and download powerpoint presentations on squash cytology ppt.
Learn vocabulary, terms, and more with flashcards, games, and other study tools. Introduction the best morphologic presentation obtained from any cytologic specimen requires an understanding of the factors that went into collecting and preparing the specimen. Stunning science powerpoint template made in orange colors and showing cells will fit presentations on cytology, cell research, bacteriology, virology, etc. This book has been prepared for students of the biological sciences who desire a means of becoming more readily acquainted with the literature and problems of cytology. Manual for cytology pdf 44p this book covers the following topics. The aim of the present study is to assess the diagnostic efficacy of squash smear cytology of fiberoptic bronchoscopic biopsies and to compare this with standard paraffin embedded sections and sputum cytology. Intraoperative squash smear cytology in cns lesions. Each of the fundamental tissues is formed by several types o. The cytohistological correlation of all 118 lesions diagnosed on cytology was 89. One of the most important concept in biology is that a cell is a basic structural and functional unit of living organism.